【摘 要】
：
目的:研究不同浓度结缔组织生长因子(connective tissue growth factor,CTGF)对人牙周膜干细胞增殖和分化的作用及相关机制.方法:组织块培养法获得原代人牙周膜细胞,通过有限稀释法和流式筛选获得人牙周膜干细胞.设实验组A、B、C、D四组(对人牙周膜干细胞培养中加入25ng/ml、50ng/ml、75ng/ml、100ng/ml外源性CTGF),对照组NC(等量溶媒),在
【机 构】
：
同济大学附属口腔医院 上海市口腔医院
论文部分内容阅读
目的:研究不同浓度结缔组织生长因子(connective tissue growth factor,CTGF)对人牙周膜干细胞增殖和分化的作用及相关机制.方法:组织块培养法获得原代人牙周膜细胞,通过有限稀释法和流式筛选获得人牙周膜干细胞.设实验组A、B、C、D四组(对人牙周膜干细胞培养中加入25ng/ml、50ng/ml、75ng/ml、100ng/ml外源性CTGF),对照组NC(等量溶媒),在第1天、3天、5天、7天使用CCK8法检测细胞增殖率,在第3天、7天通过qRT-PCR检测COL-I相关基因的表达.
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