【摘 要】
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In order to further study the biological activity of LBP3 (A sub-fractions of Lycium barbarum polysaccharide),Fluorescence labeling of LBP3 and their stability in biological samples were studied.LBP3
【机 构】
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Key Laboratory of Environmental Medicine and Engineering of Ministry of Education, and Department of
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In order to further study the biological activity of LBP3 (A sub-fractions of Lycium barbarum polysaccharide),Fluorescence labeling of LBP3 and their stability in biological samples were studied.LBP3 was labeled by fluorescein of FITC with carbon acylation reaction to obtain FITC-LBP3.The labeling efficiency was evaluated by HPGPC method and the labeling rate of FITC in FITC-LBP3 molecular was calculated as 1.3% according to the calibration curve based on the FITC standards; FT-IR analysis indicated that FITC had successfully connected to LBP3 molecules; The stability of FITC-LBP3 in vitro and in vivo was investigated using HPGPC and fluorescence spectrophotometry method with fluorescent labeled FITC-LBP3,after 24h incubation in phosphate buffer,rat blank plasma and urine at 37℃,the molecular weight and the width of molecular weight distribution were not changed,that showed FITC-LBP3 was stable in vitro.After oral administration of FITC-LBP3 to rats,FITC-LBP3 was in its original form in rat plasma and rat feces,meanwhile after an intravenous administration of FITC-LBP3 to rats,FITC-LBP3 was also excreted in its original form in rat urine,that demonstrated FITC-LBP3 metabolized in its original form.
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