The experimental study for efficacy and safety of pancreatic cryosurgery

• 作 者: David Chiu Yize Hu Zuofang Hao Kecheng Xu Lizhi Niu Feng Mu Xiang Peng Liang Zhou Haibo Li Rongrong Li Jiazhang Ni Ningzi Jiang
• 机 构: Fuda Cancer Hospital at Guangzhou,China;The GIBH Affiliated Fuda Hospital,Chinese Academy of Science
• 出 处: 国际(广州)癌症治疗论坛
• 发表日期: 2013年4期
• 关键词: Pancreatic cancer Cryosurgery Efficacy Safety Experimental study

　　Objective: This study was designed to basic information concerning the efficacy and safety of cryosur-gery for pancreatic cancer.Fifteen healthy pigs were used to perform biochemical analysis and histo-logical assessment.Methods: Following anesthesia and laparotomy, an argon-helium cryoprobe was inserted into the pancreas.The introduction of argon gas induced a rapid decrease in temperature to-160 ℃ (Group Ⅰ, 5 pigs) or-110 ℃ (Group Ⅱ, 5 pigs), respectively, resulting in ice-ball formation of 15-20 mm diameter after 5 min.Following freezing, helium gas was circulated in the probe tip to increase the temperature to 10-20 ℃over 3 min to thaw.The freeze/thaw cycle was then repeated.Group Ⅲ (3 pigs) had a cryoprobe inserted, but without freezing, and Group Ⅳ (2 pigs) included untreated or normal control animals.Levels of serum amylase (AMY), IL-6 and C-RP were measured prior to freezing and for 7 days following the procedure.All pigs were euthanized 7 days post-treat-ment and pancreases were examined histologically.Results: Neither hyperaemia, edema or hemorrhage were observed in the un-frozen parts of the pancreas.Histological assessment revealed a significant level of necrosis in the central and lateral regions of the tissue frozen within the ice-ball.All cellular ultrastructure was destroyed and only observable as a few of remaining nuclei with broken crests and degranulated mitochondria and rough endoplasmic reticulum.There was a significant increase of serum AMY levels for a brief period in both "deep frozen" and the "shallow frozen" groups.However, the AMY also increased in two pigs in the "normal control" group and one pig from the "inserted cryo-probe without freeze" control group.All experimental pigs appeared healthy until the sacrifice time.Conclusion: Cryosurgery is a safe and effective ablative procedure for pancreatic tissue resulting in min-imal complications.