Qualitative and Quantitative Analysis of Fructus Gardeniae in Ethanol Extract by HPLC-DAD-ESI-MS and

• 作 者: SuhuiLou[1]YuanyuanXie[2]YimingWang[2]QionglinLiang[2]GuoanLuo[3]
• 机 构: School of Pharmacy, East China University of Science and Technology, Shanghai, 200237, China
• 出 处: 第2届大连国际色谱学术报告会、第37届国际高效液相色谱及相关技术会议、第18届全国色谱学术报告会
• 发表日期: 2011年期

　　Gardenia is the fructus of Gardenia jasminoides Ellis which mainly grow in the provinces of Zhejiang, Jiangxi, Hubei, Hunan and Sichuan.Gardenia can be used both as medicine and food, and commonly be used to treat jaundice hepatitis, bruise, hypertension, diabetes and so on.In this paper, accurate molecular weight and fragments of compounds were obtained respectively with HPLC-DAD-TOF-MS and HPLC-DAD-TRAP-MS in ethanol extract of Gardenia.12 compounds were identified by comparing of retention times, UV spectra, and mass spectra with authentic compounds, as well as summarized MS fragmentation rules.They were Shanzhiside, Gardenoside, Genipin-l-Gentiobioside, Geniposide, Chlorogenic acid, Quercetin-3-Rutinoside, 4-O-[(E)-p-Coumaroyl]-Gentiobiosylgenipin, 3,4-Di-O-Caffeoylquinic acid, 4-Sinapoyl-5-Caffeoylquinic acid, Crocin Ⅰ, Crocin Ⅱ and Crocin Ⅳ.