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CA16 as well as Human enterovirus 71(EV71)are two major pathogens of HFMD worldwide.To characterize the antibody responses against CA16,five CA16 VP1 antigens including full length CA16 VP1(VP1 1-297),common enterovirus cross-reactive sequence(CR)of 45-58 amino acids(aa)in VP1(VP1 45-58),CR containing N terminal 1-60 aa(VP1 1-60),CR containing C terminal 41-297 aa(VP1 41-297),and 61-297 aa without CR(VP1 61-297)were constructed and analyzed by ELISA and inhibitory ELISA.