NtKRP,a Novel Kinesin-12 Protein,Specifically Regulates EmbryoSeed Size and Seed Germination Via Mod

• 作 者: Shujuan Tian Jingjing Wu Fen Li Jianwei Zou Yuwen Liu Bing Zhou Yang Bai Meng-Xiang Sun
• 机 构: CollegeofLifeSciences,StateKeyLaboratoryofHybridRice,WuhanUniversity,Wuhan430072,China
• 出 处: 湖北省细胞生物学学会2015年会员代表大会暨学术研讨会
• 发表日期: 2015年10期
• 关键词: kinesin embryogenesis cell cycle seed size tobacco

　　Kinesins comprise a superfamily of microtubule-based motor proteins involved in essential processes in plant development,but few kinesins have been functionally identified during seed development.Especially,kinesins that regulate cell division during embryogenesis have yet to be identified.Here we report the functional characterization of NtKRP,a novel motor protein of the kinesin-12 family.NtKRP is predominantly expressed in embryos and embryonic roots.NtKRP RNAi lines displayed reductions in cell numbers in the meristematic zone,in embryonic root length,and in mature embryo and seed sizes.Furthermore,we show that CDKA;1 binds to NtKRP at the latters phosphorylation sites and that the decreased cell numbers in NtKRP-silenced embryos are due to a delay in cell division cycle at the G2/M transition.