Regulation of spacer length and ligand density for improved vaccine purification by hydrophobic inte

• 作 者: LanZhao[1]BeiFan[2]YongdongLiu[3]GuanghuiMa[3]ZhiguoSu[3]
• 机 构: National Key Laboratory of Biochemical Engineering,Institute of Process Engineering,Chinese Academy
• 出 处: The 5th Sino-US joint Conference of Chemical Engineering(第五届
• 发表日期: 2009年期

　　Thioalkyl agaroses are a series of hydrophobicinteraction chromatography (HIC) adsorbents exhibiting a special selectivity in purification of macromolecules.To improve purification of rHBsAg, a vaccine molecule from CHO cells, a type of thioalkyl agarose, butyl-S-QZT 6FF, is synthesized and applied in the separation.The adsorbent is prepared by two steps: (i)activating cross-linked agarose beads with epoxides; (ii) coupiing butyl groups to the activated gel.