Cooperativity as an important organizing principle in chemistry and biology is widely utilized throughout three life kingdoms and endows the specialized functions in various complex molecular systems
For proteome analysis,two-dimensional high performance liquid chromatography coupled with tandem mass spectrometry(2D-HPLC-MS/MS)has been considered as one of the most popular approaches,by which seve
High efficiency of protein enrichment in a complex matrix is influenced by many factors.Surfactants,organic solvents as well as salts were always problematic in proteins enrichments.