论文部分内容阅读
Objective Protein kinase C (PKC) is involved in intra-cellular signal transduction in various physiological and pathological processes including substance abuse.In the present study, the role of PKC in morphine-induced rewarding memory was investigated using the conditioned place preference (CPP) model.Methods CPP was induced by different doses of morphine (0-10 mg/kg, i.p.).To observe the effect of PKC on the development of CPP (induced by 3 mg/kg morphine, i.p.), microinjection cannulas were bilaterally implanted in NAc shell and PKC translocation inhibitor γV5-3 was infused before the conditioning of morphine-induced CPP.Results (1) We found a significant translocation of PKCs from cytosol to membrane component in nucleus accumbens (NAc) of morphine-conditioned rats in a dose-dependent manner.The translocation was reduced gradually with the maintenance of morphine-induced CPP.Specifically, the protein level of PKCγ in membrane of the NAc was increased in morphine CPP rats, and decreased during the attenuation of morphine-induced CPP, while the protein level of PKCγ in cytosol of the NAc showed an opposite change.(2) The PKC translocation inhibitor γV5-3 impaired the morphine-induced CPP when microinjected into the NAc.Conclusion These findings indicated that PKC, especially the γ isoform, is essential for the acquisition and maintenance of morphine associated reward memory.