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Background: The orchid has been one of the most economic floricultural crops in China.Among them, Phalaenopsis spp.takes first place in commercial value in the orchid industry.However, viral infections result in the great reduction in both quality and quantity of flowers, hence becoming a limiting factor in the orchid industry.Cymbididum mosaic virus (CymMV) is one of the most prevalent plant viruses in orchids.The aim of this research is to generate CymMV-resistant Phalaenopsis amabilis overexpressing either the sense or anti-sense strand of the CymMV coat protein (CP) genes using particle bombardment.Hopefully, the transgenic orchids will be resistant against viral infection.Methods: Calli have been induced from P.amabilis.Plant regeneration system has been established from callus.The gene comprising the capsid proteins of CymMV has been constructed, optimum conditions for particle bombardment have been investigated.Resistant calli and transgenic plantlets have been screened on selection medium.The selection medium was optimized for shoot induction with L-methionine sulfoximine (MSO) added as selection agent.Selection was done in both solid and liquid medium.Results: It was found that liquid media and experiment showed that selection in liquid medium was faster and more efficient.PCR amplification of the inserted CP genes were used for genotyping, and PCR Southern assay confirm the insertion of anti-sense strand of CymMV CP genes into several lines of P.amabilis transformants.Northern blot and Western blot have also been done for the putative transformed plants, and viral inoculation and detection will be performed on the transgenic Phalaenopsis when they grow to be big.Conclusions: Our results showed that the transgenic P.amabilis has been successfully created, and hopefully, the transgenic orchids will be resistant against viral infection .