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The endoxylanase XT6 secreted by Geobacillus stearothermophilus is a particularly attractive candidate for some industrial purposes. The gene was de novo synthesized with the codons adjusted to fit the of that of Escherichia coli and constructed into vector pET28a(+). After optimizing the expression conditions，functional xylanase XT6 was over expressed in E.coli with up to 65％of total protein. A maximum xylanase activity of 3,030U/mL was obtained from cell extract against birchwood xylan. The recombinant XT6 was characterized. This is the first report on the over expression of a de novo synthesized xylanase XT6 gene Geobacillus stearothermophilus.