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Vitamin C, Vitamin E and NADH are three important bio-redox cofactors in vivo, they can all take place as antioxidants.Since anti-oxidation process often involves hydrogen atom transfer and the molar free energy changes of Vitamin C, Vitamin E and NADH to release hydrogen atom is vitamin E > Vitamin C > NADH, the antioxidant ability of the three compounds should be NADH > Vitamin C > Vitamin E.But, in fact, the actual antioxidant ability of the three natural compounds is Vitamin C > Vitamin E > NADH, which produces a question that what factors make vitamin C have the greatest actual antioxidant ability among the three natural bio-redox cofactors?