【摘 要】
：
Glutamate N-methyl-D-aspartate (NMDA) receptors play a pivotal role in different forms of memory.The dysfunction of NMDA receptors contributes to the pathology of CNS disorders.To further investigate
【机 构】
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Key Lab of Brain Functional Genomics, MOE & STCSM, Institute of Cognitive Neuroscience, East China N
【出 处】
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中国神经科学学会第九届全国学术会议暨第五届会员代表大会
论文部分内容阅读
Glutamate N-methyl-D-aspartate (NMDA) receptors play a pivotal role in different forms of memory.The dysfunction of NMDA receptors contributes to the pathology of CNS disorders.To further investigate the underlying mechanisms of NMDA receptors in the brain processes, we analyzed the gene expression profiles of the hippocampus between NR2B overexpression induced memory enhancement mice (Tg mice) and the littermates.The results revealed that 249 genes were significantly affected in Tg mice, which mainly involved in neurotransmission, signal transduction, cytoskeleton, hormone activity and transcription.Interestingly, the intracellular calcium channel proteins, ryanodine receptor (RyR) 1 and 3, as well as functional related proteins such as histidine rich calcium binding protein (Hrc) and triadin 2 (Trdn) were up-regulated.Moreover, our results showed that Homer-1 c protein was also increased in Tg mice, and formed a complex with RyR protein in mice brain, suggesting that Homer-1c might be an important modulator on both intracellular calcium signaling and overall neuronal signaling by interacting with NMDA receptor and RyR simultaneously.Western blot and Real-time PCR results showed that the expression of phospho-CREB, c-fos, immediate early genes Egr2 and Egr4 were also up-regulated in Tg mice.Our studies demonstrated that chronic increased activation of NMDA receptors modified the expression of a large number of genes, which may provide important clues for better understanding the molecular mechanism of NMDA receptor modulated learning and memory and CNS disorders.
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