Establishment of Latent Infection and Drug-induced Reactivation of Oncolytic Herpes Simplex Virus in

• 作 者: TiborValyi-Nagv;
• 机 构: Associate Professor and Director of Neuropathology University of Illinois at Chicago USA
• 出 处: 2011第四届世界癌症大会
• 发表日期: 2011年期

　　To determine whether herpes simplex virus type-1 (HSV-1) can establish latent infection in tumor cells, two-dimensional (2D) monolayer cultures ofOCM-1 uveal melanoma cells were inoculated with a recombinant HSV-1 strain (K26GFP) that expresses the green fluorescent protein (GFP) upon replication.Cells of the monolayers were scraped off one hour after virus inoculation and were mixed with extracellular matrix (Matrigel)-containing media to establish threedimensional (3D) tumor cultures.We found that some HSV-l-inoculated cells expressed GFP and were destroyed in the 3D cultures.