【摘 要】
：
Objective: we aimed to investigate whether the expression and functional activity of RyRs would be different in mesenteric artery from patients with hypertension.Methods: RyR expression, [Ca2+]i were
【机 构】
：
Key Laboratory of Medical Electrophysiology,Ministy of Education of China,and the Institute of Cardi
【出 处】
：
International Conference for Physiological Sciences 2012(201
论文部分内容阅读
Objective: we aimed to investigate whether the expression and functional activity of RyRs would be different in mesenteric artery from patients with hypertension.Methods: RyR expression, [Ca2+]i were measured by real-time quantitative RT-PCR, immunofluorescence staining and wide-field fluorescence microscopy, respectively.Results: All 3 RyR subtype (RyR1 RyR2 and RyR3) mRNAs are expressed in HMASMCs, but their expression levels and distributions are different.RyR1 and RyR3 localize mostly to the central sarcoplasmic reticulum (SR),while RyR2 are especially the predominant isoform and distribute around the plasma membrane, colocalizing with BKCa channels.The expression level of RyR2 is significantly decreased in patients with hypertension.Activation of RyRs by caffeine (10 mM) or high K+ (60 mM) induces differential Ca2+ release.Moreover, the increases and decreases in [Ca2+]i induced by caffeine is faster in HMASMCs from patients without hypertension relative to those with hypertension.Conclusion: This study provides comprehensive evidence that RyRs are heterogeneous in expression and functional activity in HMASMCs from patients with hypertension, which may contribute to the diversity of excitation-contraction coupling and intimate communication between SR and sarcolemmal ion channel in different vascular smooth muscle cells.
其他文献
Objective: To investigate the aerobic exercise-induced remodeling of the cardiovascular responses and mesenteric arterial function of aged rats, and to explore the possible mechanisms.Methods: Old mal
Objective: To observe the role of activation of aldehyde dehydrogenase 2 (ALDH2) on myocardial ischemia/reperfusion injury in diabetic rats, and investigate the related mechanism.Methods: Diabetic rat
Monocyte-derived macrophage migration and foam cell trapping are the main events in the progression of atherosclerosis.Tropomodulin1 (Tmod1), a capping protein at the pointed-end of actin filaments, i
Objective: Physical inactivity and ageing are widely recognized as risk factors for the development of cardiovascular diseases such as hypertension.Our recent studies has demonstrated that there is a
Safrole-2,3-oxide (SFO) is the electrophilic metabolite of safrole, a natural plant constituent found in oils of numerous edible herbs and spices.The effects of SFO in cardiovascular system are little
Objective To observe the effect of endurance training on Mfn2 protein expression and mitochondrial function, so as to study the mitochondrial mechanism that endurance training elevated aerobic capacit
To investigate whether hydrogen sulfide (H2S) protect the heart against myocardial ischemia/reperfusion (I/R) injury by inhibiting apoptosis caused by I/R-induced excessive endoplasmic reticulum stres
Objective To observe changes in mitochondrial fusion of skeletal muscles in rats after hypoxia and hypoxia combined with training, in order to understand the effects of proper training under hypoxia o
Objective: To investigate the effect of sodium tanshinone Ⅱ-A sulfonate (DS-201) on large conductance Ca2-sensitive K+ channels (BKCa) and to further study by focusing on the kinetics of DS-201 on BKC
Objective: The aim of the present study was to study the effect of estrogen on the large-conductance Ca2+-activated potassium (BKCa) channel currents and spontaneous transient outward currents (STOCs)