MiR-342-5p suppresses coxsackievirus B3 replication by targeting 2C-coding region

• 作 者: Ying Qin Lexun Lin Lei Tong Shuo Wu Zhiwei Guo Wenran Zhao Zhaohua Zhong
• 机 构: DepartmentofMicrobiology,HarbinMedicalUniversity,Harbin150081,ChinaDepartmentofCellBiology,HarbinMed
• 出 处: 中国微生物学会2012感染与免疫学术研讨会
• 发表日期: 2012年4期
• 关键词: MiR-342-5p Coxsackievirus B3 2C-coding region Antiviral activity

　　Coxsackievirus B type 3 (CVB3) is one of the main pathogens of myocarditis and cardiomyopathy.MicroRNA is a class of short noncoding RNA which can post-transcriptionally modulate gene expression.By comparing the sequences of CVB3 genome and miR-342-5p, we found that there were potential targets of miR-342-5p in the CVB3 genome.In HeLa cells infected with RLuc-CVB3, a CVB3 variant which expresses Renilla luciferase (RLuc), miR-342-5p could significantly inhibit RLuc expression while miR-mock and other 5 miRNAs could not.In HeLa cells infected with EGFP-CVB3, a CVB3 variant which expresses EGFP, the EGFP expression was significantly inhibited by miR-342-5p.