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Langmuir-Blodgett (LB) phospholipid monolayers at the air-water interface are useful model membranes to understand miscibility and molecular interaction among various components.Based on the regular solution theory,the thermodynamic behavior and the miscibility of the two lipids in the mixed monolayer were evaluated in terms of the excess mean molecular area,excess Gibbs free energy and compressibility coefficient.1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) and 1,2-dioleoyl-3-trimethylammonium-propane (DOTAP) were miscible and there was repulsive interaction between DMPC and DOTAP molecules for most of selected ratios and surface pressures.