Effects of target gene expression on ex vivo differentiated mesenchymal stromal cells transfected by

来源 :泌尿生殖肿瘤华夏医学论坛2016暨长城国际泌尿男科转化医学论坛2016 | 被引量 : 0次 | 上传用户:teruiandi
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  Objective: To investigate the expression of PDE5 in ex vivo differentiation of gene-modified BM-MSCs using the lentiviral vector containing silencing target gene PDE5 of shRNA.Methods: SD rat bone marrow-derived MSCs were separated,cultured and purified in vitro by Percoll density gradient centrifugation combined with adherent culture.
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