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Monolayer ultra-large graphene oxide (UL-GO) sheets with sizes up to ~100 μm are synthesized based on a chemical method.Transparent conductive films are produced using the UL-GO sheets that are deposited layer-by-layer on a substrate using the Langmuir-Blodgett (L-B) assembly technique.The films produced from UL-GO sheets with a close-packed flat structure exhibit exceptionally high electrical conductivity and transparency after thermal reduction.A remarkable sheet resistance of 605 Ω/sq at 86% transparency is obtained, which outperforms the graphene films grown on a Ni substrate by chemical vapor deposition.The technique used in this work to produce transparent conductive UL-GO thin films is facile, inexpensive and tunable for mass production.