Engineering dual-luciferase reporter toolkits for optimizing nucleic acid-guided nucleases for gene

来源 :中国化学会第十届全国化学生物学学术会议 | 被引量 : 0次 | 上传用户：huangshuhui1983

【摘要】

：

CRISPR/Cas9 is a newly-developed technology for gene intervention,which relies on a single guide strand called sgRNA to exhibit its DNA cleavage activity.

【作者】

：

Zhen Xi

【机构】

：

State Key Laboratory of Medicinal Chemical Biology and College of Life Sciences,Department of Chemic

【出处】

：

中国化学会第十届全国化学生物学学术会议

【发表日期】

：

2017年10期

【关键词】

：

CRISPR/Cas9 Dual luciferase report system

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　　CRISPR/Cas9 is a newly-developed technology for gene intervention,which relies on a single guide strand called sgRNA to exhibit its DNA cleavage activity.

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Engineering dual-luciferase reporter toolkits for optimizing nucleic acid-guided nucleases for gene

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