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This study presents a soil moisture assimilation scheme, which could assimilate microwave brightness temperature directly, based on the ensemble Kalman filter and the shuffled complex evolution method (SCE-UA).It uses the soil water model of the land surface model CLM3.0 as the forecast operator, and a radiative transfer model (RTM) as the observation operator in the assimilation system.The assimilation scheme is implemented in two phases: the parameter calibration phase and the pure soil moisture assimilation phase.