【摘 要】
：
Rapeseed (Brassica napus) is one of the most important edible oilseed crops in the world and is increasingly used globally to produce bio-diesel.Therefore, increasing oil content of oilseed corps is o
【机 构】
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Key Laboratory for Biological Sciences of Oil Crops,Ministry of Agriculture,Oil Crops Research Insti
【出 处】
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中国作物学会油料作物专业委员会第七次会员代表大会暨学术年会
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Rapeseed (Brassica napus) is one of the most important edible oilseed crops in the world and is increasingly used globally to produce bio-diesel.Therefore, increasing oil content of oilseed corps is of importance economically in both food and oil industries.The wri1 genes are differentially expressed in B.napus lines with different oil content.To investigate the effects of B.napus WRI1 (BnWRI1) on oil content, two Bnwril genes with different lengths, Bnwril-1 and Bnwril-2, were identified and sequenced.Homology analysis shows 80% amino acids of Bnwril s are homologous to Arabidopsis thaliana WRI1 (AtWRI1).Overexpression of Bnwril cDNAs driven by cauliflower mosaic virus 35S-promoter in 51 transgenic A.thaliana lines resulted in 10-40% increased seed oil content and enlarged seed size and mass.Detailed analysis on transgenic embryos indicates an increased cell size other than cell number.In addition, Bnwril sequence polymorphism is highly related to oil content (p <0.001).Taking together, Bnwril has potential applications in food and oil industries and in rapeseed breeding.
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