【摘 要】
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The homeostasis of pH is crucial in cell physiology.Developing small-molecular fluorescent probe for the ratiometric detection of pH fluctuation is highly demanded yet challenging.1 A novel probe resp
【机 构】
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College of Chemistry and Chemical Engineering,Henan University,Kaifeng,475004,China
【出 处】
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中国化学会第十六届全国有机合成化学学术研讨会
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The homeostasis of pH is crucial in cell physiology.Developing small-molecular fluorescent probe for the ratiometric detection of pH fluctuation is highly demanded yet challenging.1 A novel probe responding to both acid and alkali has been developed.The ionizations of hydroxyl at the coumarin 7-position and nitrogen atom at the indole causeing the extreme alkali response,which leads to the intense fluorescence quenching of probe S1(3.33× 10-5 mol/L)in DMSO/HEPES buffer solution(1:1 v/v)at 650 nm.Moreover,the color of the probe S1 solution also changes from red to blue.Under acidic conditions,a significant red to yellow change was observed accompanying a change in fluorescence intensity,which contribute to the addition reaction mechanism between hydrogen ion and carbon-carbon bond.The fluorescent imaging in bacterial cells under extreme pH conditions supports the mechanism of pH response.2 The fluorescence change of probe S1 is reversible and takes place mainly with in the pH range from 1.3 to 7.0 and 7.0 to 13.0,which is valuable for the detection of strong acidic or alkaline environment.
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