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Aim.Shigyaku-san is known to alleviate the effects of experimental liver damage.The aim of this study was to clarify the effects of Shigyaku-san on liver function and regeneration after hepatectomy in rats.Methods.Rats were divided into three groups (control, Shigyaku-san, no-hepatectomy).Blood biochemistry was analyzed at 2, 6, 24, and 72 h postoperatively.The remnant liver, liver-body weight ratio and immunohistochemistry stains were evaluated before hepatectomy and at 24 and 72 h postoperatively.Serum IL-6 and TNF-α were measured before hepatectomy and 2 and 6 h postoperatively.Results.Serum ALT and T-Chol were significantly lower in the Shigyaku-san group than in the control group at 24 and 72 h postoperatively, and serum LDH was significantly lower 6 h postoperatively.Regenerated liver weight was significantly increased in the Shigyaku-san group compared with the control group at 72 h postoperatively.The proliferating cell nuclear antigen (PCNA) labeling index (LI) and liver-body weight ratio were significantly higher in the Shigyaku-san group than in the control group 24 and 72 h postoperatively.Ki67 was significantly higher in the Shigyaku-san group than in the control group 72 h postoperatively.Compared with the control group, serum IL-6 was significantly increased 2 h postoperatively, but significantly decreased 6 h postoperatively in the Shigyaku-san group.Conclusion.These data suggest that Shigyaku-san protects against liver damage and promotes liver regeneration after partial hepatectomy in rats.