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Lesions to the fimbria fornix (FiFx) plus cingulate bundle (CB), the principal routes of communication of forebrain cholinergic regions, produce lasting impairment of spatial learning and memory in mice.We report that extensive neurogenesis takes place in the FiFx, CB and basalis magnocellularis (MBN) following FiFx plus CB transection.Immunofluorescence revealed that nestin-expressing cells were present in all three areas following lesion; the majority of nestin-positive cells were also positive for 5-bromo-2-deoxy-uridine (BrdU) incorporation, a marker of DNA synthesis.Nestin-positive proliferative cells were almost entirely absent from unlesioned tissue.Neurospheres cultured in vitro from lesioned FiFx displayed the characteristics of neural stem cells (NSCs)-proliferation, expression of embryonic markers, and multipotential differentiation into neurons, astrocytes and oligodendrocytes.At early stages after transection a small number of immature and migrating doublecortin (DCX)-immunopositive neurons were detected in lesioned FiFx where neuronal cell bodies are normally absent.At later stages post-lesion immature neurons developed into β-tubulin Ⅲ-positive mature neurons.Lentivirus labeling assay implied that the injury-induced neurogenesis in FiFx may be from local neurogenic astrocytes but not from dentate gyrus.These results demonstrate that insult to cholinergic tracts can stimulate NSC proliferation and neuronal regeneration not only in innervated regions but also in the projection pathways themselves.Ectopic neurogenesis in cholinergic system-related areas provides an additional mechanism for repair of cholinergic innervation following damage.