Highly Specific Peptide Probes for the Activatable Bioimaging and Targeted Tracing of Tumor Markers

• 作 者: Yanyan Huang Rui Zhao
• 机 构: BeijingNationalLaboratoryforMolecularSciences,CASKeyLaboratoryofAnalyticalChemistryforLivingBiosyste
• 出 处: 第九届全国微全分析系统学术会议、第四届全国微纳尺度生物分离分析学术会议、2014国际微流控芯片与微纳尺度生物分离分析学术
• 发表日期: 2014年10期
• 关键词: peptide affinity screening cancer bioimaging

　　Smart molecular probes capable of sensitive,dynamic and multiparameter sensing of molecular changes in complicated biosystems are promising for cancer diagnosis and therapy.To this end,functional evolution of cancer-specific peptides was carried out by the combination of antisense peptide approach and affinity chromatographic screening.An optimal peptide AP2H was identified with high affinity and specificity towards a novel cancer biomarker LAPTM4B protein.By incorporating an aggregation-induced-emission(AIE)fluorophore,a fluorescence turn-on bioprobe(AP2H-TPE)was successfully constructed.The fluorescence signal of AP2H-TPE can be proportionally switched on by the target protein and responsive to the environmental pH as well.Capable of discriminating the expression level of LATM4B protein,AP2H-TPE allows highly specific bioimaging of cancer cells.Real-time monitoring and subcellular localization of LAPTM4B in cancer cells were facilely acquired with a very high target-to-background ratio.Taking advantage of the targetable recognition of AP2H,a dual-functional red-emissive peptide probe TPEred-2AP2H was further developed for the simultaneous fluorescence turn-on bioimaging and photodynamic therapy of tumor cells.Such highly selective peptide probe for molecular signatures of cancer cells can be potential in cancer diagnosis and targetable drug delivery.