Determination of mangiferin and homomangiferin in the leaves of manglferainidca L.from 93 cultivated

• 作 者: XuFeng;ShengboWang;JiepingQin;JiagangDeng;
• 机 构: Guangxi Traditional Chinese Medical University, Nanning ,P.R.China
• 出 处: 农作物废弃物功能成分筛选暨首届芒果苷研究国际研讨会
• 发表日期: 2009年期

　　To establish a method for the determination of mangiferin and homomangiferin in the leaves of Mangifera indica L by HPLC.The separation was performed on a Elite Hypersil C18 column (5um,4.6mmIDX250mm),with a gradient system of acetonitrile-0.1%H3PO4 as mobile phase at a flow rate of 1.0 mL/min.The detective wavelength was 258nm,the column temperature was 30℃.The linear ranges of mangiferin and homomangiferin were good within the range of 0.0254-0.508ug/uL(r=0.9999) for mangiferin and 0.000960-0.0192ug/uL(r=0.9999) for homomangiferin.The average recovery rate were 101.7% (RSD=2.0%),101.0%(RSD=1.7%).The content of Mangiferin and homomangiferin in the leaves of Mangifera indica L.were very different due to the different cultivated varieties.It can further improve the quality control system of the leaves of Mangifera indica L.