Genetic diversity of Erodium Stephanianum Willd.based on ISSR analysis

• 作 者: YINHai-bo;
• 机 构: School of Pharmacy, liaoning University of Traditional Chinese Medicine, Dalian 116600, china
• 出 处: 世界中医药学会联合会中药鉴定专业委员会成立大会暨第一届学术年会
• 发表日期: 2014年期

　　Objective: This article assessed the genetic relationship and genetic diversity in Erodium Stephanianum Willd..Method: 23 germplasmic resources of Erodium Stephanianum Willd.Were analyzed by ISSR molecular markers.Genetic similarities were calculated by using NTSYS software and the dendrogram was constructed by using UPGMA method..Result: Fourteen ISSR primers were selected from 100 ISSR primers, and 185 DNA fragments were amplified from 23 samples.Of which, 142 fragment were polymorphic (percentage of polymorphic bands was 76.8%).The genetic similarity among all accessions ranged from 0.458 to 0.916.Clustering analysis showed that the 23 samples of Fritillaria could be distinctively classified into 6 groups.Conclusion: Results from the cluster analysis were correlated significantly with the morphological characteristics and geographical location of 23 samples.The data indicate that ISSR technique is useful to determine genetic diversity and genetic relationship among Erodium stephanianum Willd.provenances, providing a scientific basis for genetic breeding, differentiation and new cultivar selection.