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The occurrence of pharmaceutical and personal care products (PPCP’s) in the environment water is of growing concern due to their potential harmful effects on environmental and human.The conventional methods based on the “template” of EPA 1694 methods require long and pre-concentration of large volume of water sample and tedious SPE cleanup,followed by LC-tandem mass spectrometer analysis in order to achieve the low ng/L (ppt) level detection.In this study,we introduce an UHPLC system with an integrated online extraction option coupled to triple quadrupole mass spectrometer for detection of PPCPs in water.The built-in online extraction requires no additional hardware assembly (LC pump and valve),and can readily detect 1-5 ppt of PPCPs in water by rapidly pre-concentrating ~0.5 mL water sample.More than 15 PPCP’s were studied.The study was carried out on an EVOQ Elite model triple quadrupole mass spectrometer coupled to Advance UHPLC OLE system consisting of an integrated third pump (the other two for binary gradient separation) for sample preparation and a 10-port valve.The sample was injected via the loop and loaded onto an trap column,after cleanup with the use of third pump,the targeted PPCP’s were back flushed onto the analytical column for separation with a binary gradient.The EVOQ was operated in ESI mode with both positive and negative polarities depending upon the target compounds.The polarity of the ESI source,injection volume,chromatographic conditions,and breakthrough volume of the trap column,etc were optimized.The results showed that most of the PPCP’s can be detected with 1-5 ppt or better with the injection of 0.5 mL water samples,with a linear response range (R2>0.99) up to 100-500 ppt.Replicate injections with 5 ppt level spiked in tape water demonstrate good robustness with RSD of 5-15% (n=10).