【摘 要】
：
Objective Immune thrombocytopaenia (ITP) is characterized by platelet destruction and megakaryocyte dysfunction resulting from autoantibodies induced by the polarization of Th1 and deficient regulator
【机 构】
：
北京大学人民医院 北京大学血液病研究所
论文部分内容阅读
Objective Immune thrombocytopaenia (ITP) is characterized by platelet destruction and megakaryocyte dysfunction resulting from autoantibodies induced by the polarization of Th1 and deficient regulatory T cells.Mesenchymal stem cells (MSCs) display not only stem cell multipotency but also robust immunosuppressive properties.However, MSCs from ITP patients do not exhibit conventional proliferative abilities and thus exhibit defects in immunoregulation, while the transplantation of health MSCs could improve platelet production and attenuate megakaryocytedysfunction by reversing the shift in the Th1/Th2 cytokine balance, suggesting that MSC impairment might be a mechanism involved in ITP.In addition, reversal of the defects in MSCs may represent a potential therapeutic alternative.Platelet-derived growth factor (PDGF) improves growth and survival in various cell types.Moreover, PDGF promotes MSCs proliferation.
其他文献
目的 免疫性血小板减少症(ITP)是一种自身免疫性出血性疾病,T细胞免疫调控在ITP的发生发展中起重要的作用.我们前期研究发现ITP中存在异常的TCR亚家族克隆增殖和TCR信号通路,本研究进一步分析近期所报道的发挥T细胞免疫耐受作用的T细胞免疫抑制受体程序性死亡分子1 (PD1)及其配体PD-L1的表达情况.方法 利用实时定量PCR检测18例初诊ITP病人外周血单个核细胞中PD-1和PD-L1基因
目的 异常活化炎症调控因子核转录因子-κB (NF-κB)可引起细胞过度增殖,分化障碍和凋亡抑制,导致自身免疫性疾病和淋巴造血系统肿瘤发生的重要原因.而粘膜相关组织淋巴瘤异位基因1 (MALT1)是NF-κB信号通路中重要因子,其异常表达、突变及基因重排与血液系统肿瘤的发生相关.前期研究发现淋巴细胞白血病中MALT1基因相关信号通路表达异常.本研究通过构建慢病毒MALT1两种异构体表达载体,分析上
目的 微小RNA(microRNA,miRNA)是近几年在真核生物中发现的一类内源性的具有调控功能的非编码单链小分子RNA,其大小长约19-23个核苷酸,能够以碱基不完全互补的方式与靶基因结合,在转录后水平上对靶基因进行负向调控.作为重要的调节分子,miRNA参与了充质干细胞(MSCs)的增殖、分化、迁移以及细胞因子的分泌等一系列重要的生理和病理过程.本研究利用miR-155抑制剂或其类似物转染骨
探讨小鼠经异基因骨髓移植后,发生移植物抗宿主病(GVHD)时体内IL-22的细胞来源.分别以C57BL/6和BALB/c小鼠为供受鼠,受鼠经致死剂量全身照射后输注供鼠骨髓和脾淋巴细胞悬液,建立GVHD模型.实验分为正常组(Normal)、单纯照射组(TBI)、骨髓移植组(BMT)和骨髓联合脾细胞诱导GVHD组(BS).ELISA法检测小鼠血浆中IL-22的水平；流式细胞术检测IL-22的细胞来源及
Objective To further investigate the role of Treg cells in ITP murine model.Methods 1.ITP murine model was induced by daily intraperitoneal injection of antiplatelet membrane CD41 antibody (MWReg30) a
Graft-versus-host disease (GVHD) remains the major obstacle for allogeneic bone marrow transplantation, whic h is considered to mediate mainly by alloreactive donor CD4+ T cells.Th22 cell as a newly i
OBJECTIVE To explore the mechanism and signal pathway of IL-22 in the pathological damage organs of GVHD mice.METHOD The tissues sections of GVHD target organs were stained wi th HE for histopathologi
INTRODUCTION Graft-versus-host disease (GVHD) as the main complication of allogeneic haematopoietic stem cell transplantation is still not solved.It is known that allogeneic reactive CD4+ T and CD8+ T
目的 骨髓增生异常综合征(MDS)是一组异质性的克隆性造血干细胞疾病,大约30％的患者发展为急性髓性白血病(AML).免疫调节异常可能促进MDS的发生和发展,在MDS患者我们已观察到T细胞受体(T cell receptor,TCR) Vβ谱系表达和克隆性扩增异常改变.为了进一步了解MDS患者γδT细胞的特征,我们研究了难治性贫血伴原始细胞过多(refractory anemia with exc
研究背景及目的 免疫球蛋白(Immunoglobulin, Ig)是B细胞转化为浆细胞产生的重要免疫效应分子,也是B细胞识别抗原的受体(BCR),这是写入国内外免疫学教材的经典概念。然而,我们曾于1996年于国内首报,2003年在国际上首报非B细胞起源的肿瘤细胞也可产生Ig、而且该Ig分子具有生长因子样活性。这一有悖免疫学经典理论的发现,历经了十几年的艰辛历程,目前已经明确,Ig分子在非B细胞来源