The 30K proteins in the hemolymph of silkworm (Bombyx mori) are a family of low molecular weight lipoproteins.The functions of these proteins are still limited at the current stage.In this short commu
Although the antiviral actions of interferons (IFNs) are first observed,up to date,Type Ⅰ IFNs (α/β) protein have been widely used as a clinical anticancer agent due to their antiproliferative effects
We previously demonstrated that serine protease inhibitor BmSPI could strongly inhibit subtilisin A.In order to characterrize the localization of BmSPI,the recombinant protein was expressed in Escheri
应用双向凝胶电泳(2-DE)技术研究家蚕孤雌生殖蚁蚕和正常蚁蚕的差异，得到一些差异蛋白点，并对这些差异蛋白点进行基质辅助激光解吸电离串联飞行时间质谱(MALDI-TOF-TOF MS)分析，获得了相关差异蛋白的序列特征。将这些序列与已构建的家蚕cDNA文库及蛋白质数据库进行比对，得到其中1个差异蛋白为磷脂酰肌醇蛋白聚糖。通过5-RACE的方法克隆了此差异蛋白的全基因序列，分析了它的结构和特征，对它
从家蚕蛹cDNA文库中获得家蚕死亡相关蛋白BmSCLP的cDNA序列.该cDNA序列长1557bp,含642bp的ORF序列,编码213个氨基酸残基.将克隆的该基因插入pET-28a构建重组质粒,转化大肠杆菌BL21后诱导表达融合蛋白,经镍柱亲和层析纯化得到较纯的目的蛋自,再通过反相快速液相色谱(RP-FPLC)进一步纯化,质谱鉴定该蛋白的相对分子质量为27.74kD,将纯化好的目的蛋白作为抗原多
We have developed a novel baculovirus surface display (BVSD) system for the isolation of membrane proteins.We expressed a reporter gene that encoded HA fused in frame with the signal peptide and trans