Preparation and Characterization of Anti-HER2 Affibody modified PE38KDEL-Ioaded PEGylated immunolipo

• 作 者: LeiZhang;YingLu;YanqiangZhong;
• 机 构: Department of Pharmaceutics,School of Pharmacy,Second Military Medical University,Shanghai 200433,Ch
• 出 处: 2012年中国药物制剂大会——中国药学会药剂专业委员会年会、亚洲阿登制药技术研讨会暨国际控释协会中国分会年会
• 发表日期: 2012年期

　　Objective:Toxin protein of immunotoxin produces a strong non-specific toxicity in the body,and greatly limits their broad clinical applications.ZHER2:342-PE38KDEL-loaded anti-HER2 PEGylated liposomes(ZP-Affisomes) were constructed.Methods:Particle size and morphology of ZP-Affisomes and ZP-liposomes were observed by dynamic light-scattering detector (DLS) and transmission electron microscope (TEM).BCA assay was used to determine the drug loading,encapsulation efficiency,and high performance liquid chromatogaphy(HPLC) was used to determine ZHER2:342 conjugation efficiency on the ZP-liposomes.