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Candida parapsilosis,which was previously considered to be a complex of three geneti-cally distinct groups,has emerged as a significant agent of nosocomial infections.Recently,this complex was separated into three species: C.parapsilosis sensu stricto,C.orthopsilosis and C.metapsilosis.In China,data pertaining to these fungi are lim-ited.In this study,we examined 57 isolates of members of the C.parapsilosis com-plex collected from four cities in East China,i.e.,Nanjing ( n=22),Nanchang (n=20),Shanghai (n=12) and Jinan (n=3).C.parapsilosis sensu stricto represented 71.9% of all isolates,while C.metapsilosis accounted for the remaining 28.1%.C.orthopsilosis could not be identifi ed.A significantly high prevalence of C.metapsilosis was observed in strains recovered from Nanchang,60% (12/20) of the isolates were C.metapsilosis.Sequence analysis of internal transcribed spacer region revealed two unevenly distrib-uted genotypes among the C.metapsilosis strains.A PCR-restriction fragment length polymorphism assay was described for rapid identification.The strains were susceptible to fluconazole,voriconazole,amphoterincin B and micafungin.Six (15%) isolates of C.parapsilosis sensu stricto and three (18.8%) of C.metapsilosis were found to be dose-dependent susceptible to itraconazole.C.parapsilosis sensu stricto strains were less susceptible to micafungin than C.metapsilosis.