Microarray analysis of differentially expressed genes in Bombyx mori Ovary, two silkworm cell lines

• 作 者: DONG Xiao-Long WANG Ting-Ting LIU Tai-Hang WANG Wei PAN Cai-Xia ZHANG Jun CHEN Peng LU Cheng PAN Min-Hui
• 机 构: State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400716, China
• 出 处: 中国蚕学会第八届青年学术研讨会
• 发表日期: 2014年10期
• 关键词: Bombyx mori Nucleopolyhedrovirus Cell line Gene expression

　　The silkworm is an important economic insect and the viral disease,especially nucleopolyhedrovirus,results in a great loss of sericulture.Many host genes were confirmed to have the activity of nucleopolyhedrovirus resistance,such as BmHSC70-4,seroin and BmNOX.In addition,some small molecular compounds,such as chondroitin sulfate and cholesterol,are also related to nucleopolyhedrovirus infection.Our lab kept two cell lines which have the different antiviral activity of nucleopolyhedrovirus,which may provided a perfect material for us to study the nucleopolyhedrovirus resistance.To investigate the molecular mechanism of silkworm resisitance to BmNPV infection,we compared the differential expressed genes in BmN vs Ovary,BmNS vs Ovary,BmN vs BmNS and the pre-/post-infection stage of the two cell lines.We found most of the differential expressed genes fell into the signal transduction pathway.We detected 104 genes which were induced expression by BmNPV,and these genes may play an important role in BmNPV resistance of silkworm cell lines.Then,9 of these 104 genes were chose to confirm by qRT-PCR,the results were similar with the microarray data.The results suggest that these different expression genes which are related to signal transduction may play important roles in BmNPV resistance.Our research suggests that the receptor or transporter may be not the only key factor of BmNPV transduction,and the signal transduction,even though,may play a more important role than the receptor or transporter in BmNPV transduction.