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Nitric oxide (NO) is a kind of low molecular weight endogenous signaling molecule which was well-known as "Molecule of the Year" in 1992 for its important effects in a number of diseases.Furthermore, there is lots of evidence that NO can improve the activity of chemotherapeutic drugs and co-delivery of NO and chemotherapy drugs will greatly suppress the growth of tumor [1].However, delivery of NO to the tumor directly is not very effective because of its short half-life time in the body.NO donors is promising way to transport NO to the targeted area.Many NO donors have been developed, including organic nitrates, S-Nitrosothiols and Diazeniumdiolates and so on.Among them, organic nitrates are nitric acid esters and the oldest NO donors used in clinic.Besides, the high stability of organic nitrates makes it easier to realize controlled release and study the logical properties of NO in cells and in vivo.Here, we reported a straightforward method to conjugate nitrate to a biodegradable and biocompatible material (D-α-tocopheryl polyethylene glycol 1000 succunate, Vitamin E TPGS or simply TPGS) which greatly improved stability of NO in aqueous.Several examinations were carried out to verify the anti-tumor activity of TPGS-NO micelle alone and the combination with doxorubicin (DOX) in vitro and in vivo.Finally, we demonstrated that co-delivery of TPGS-NO micelle enhances the anti-tumor activity of DOX, as well as its pharmacokinetic properties.