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目的探讨姜黄素对汞致大鼠肾损伤的影响,为汞中毒的发病机制和防治提供实验依据。方法 Wistar大鼠30只按体重随机分为5组,每组6只,雌雄各半。分别为对照组、低剂量染汞组、中剂量染汞组、高剂量染汞组和姜黄素预处理组。1~4组大鼠予皮下注射0.9%氯化钠溶液,第5组大鼠给予皮下注射100 mg/kg姜黄素;2 h后,第1组腹腔注射生理盐水,第2~5组大鼠分别腹腔注射2.2、4.4、8.8和8.8μmol/kg氯化汞溶液,连续干预与染毒3 d。第3天染毒2 h后将动物放入代谢笼,收集24 h尿液测定尿汞和尿蛋白含量,以及尿碱性磷酸酶(ALP)、β-N-乙酰氨基葡萄糖苷酶(NAG)和乳酸脱氢酶(LDH)活力;用乙醚将大鼠麻醉,腹主动脉采血测定血清尿素氮(BUN);切取肾皮质,测定肾皮质汞、还原型谷胱甘肽(GSH)和丙二醛(MDA)的含量及超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活力。结果与对照组比较,随着染汞剂量的增加,肾皮质汞、尿汞、尿蛋白和BUN含量均升高;尿NAG、LDH和ALP活力均升高;肾皮质GSH和MDA含量明显升高而GSH-Px和SOD活力显著下降,差异具有统计学意义(P<0.05)。姜黄素预处理组与高剂量氯化汞染毒组比较,各项指标均有不同程度的改善,差异有统计学意义(P<0.05)。结论姜黄素对汞致大鼠肾损伤具有一定的保护作用。
Objective To investigate the effect of curcumin on renal injury induced by mercury and provide experimental evidence for the pathogenesis and prevention of mercury poisoning. Methods 30 Wistar rats were randomly divided into 5 groups according to body weight, 6 in each group, with half male and one female. Respectively, the control group, low-dose mercury stain group, middle-dose mercury stain group, high-dose mercury stain group and curcumin pretreatment group. Rats in group 1 ~ 4 were subcutaneously injected with 0.9% sodium chloride solution, while rats in group 5 were given subcutaneous injection of 100 mg / kg curcumin. After 2 hours, group 1 received intraperitoneal injection of saline and rats in groups 2 to 5 Intraperitoneal injection of 2.2,4.4,8.8 and 8.8μmol / kg of mercuric chloride solution, continuous intervention and exposure to 3 d. Animals were placed in metabolic cages on the third day after exposure for 2 h. Urinary mercury and urinary protein contents were collected 24 h urine and urine alkaline phosphatase (ALP), β-N-acetylglucosaminidase (NAG) And lactate dehydrogenase (LDH) were measured. The rats were anesthetized with ether and blood was taken from the abdominal aorta to measure the serum urea nitrogen (BUN). The renal cortex was cut out to determine the renal cortical mercury, reduced glutathione (GSH) Aldehyde (MDA) content and superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activity. Results Compared with the control group, the levels of mercury, urinary mercury, urinary protein and BUN in the renal cortex increased with the increase of the dose of mercury, the activities of urine NAG, LDH and ALP increased, and the levels of GSH and MDA in the renal cortex increased significantly While the activities of GSH-Px and SOD decreased significantly (P <0.05). Curcumin pretreatment group and high dose mercuric chloride exposure group, the various indicators have improved to varying degrees, the difference was statistically significant (P <0.05). Conclusion Curcumin has a protective effect on renal injury induced by mercury in rats.