【摘 要】
：
Dicrocoeliosis,caused by D.dendriticum,is a parasitic disease in ruminant worldwide,causing severe economic losses due to liver function impairment.Traditional morphological methods for identification
【机 构】
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College of Veterinary Medicine,Northwest A&F University,Yangling,Shaanxi Province 712100,People' s
【出 处】
：
中国畜牧兽医学会家畜寄生虫学分会第七次代表大会暨第十二次学术研讨会
论文部分内容阅读
Dicrocoeliosis,caused by D.dendriticum,is a parasitic disease in ruminant worldwide,causing severe economic losses due to liver function impairment.Traditional morphological methods for identification and differentiation of D.dendriticum,are difficult to infer inter:species differences ofDicrocoelium spp.and can not indicate intra:species or intra:population differences ofD.dendriticum.In the present study,genetic variations in three mitochondrial DNA (mtDNA) regions,namely portion ofcytochrome c oxidase subunit 1 (pcoxl),NADH dehydrogenase subunit 1 (pnadl) and cytochrome b (pcytb),were examined for Dicrocoelium dendriticum samples isolated from different origins in Shaanxi province,northwestern China.The intra:specific sequence dif-Mples isolated from different origins in Shaanxi province,northwestern China.The intra:specific sequence differences within D.dendriticum samples were 0:0.52% for pcoxl,0:0.73% for pnadl,and 0:0.58% for pcytb.Phylogenetic analyses based on combined sequences of three mtDNA showed that all D.dendriticum sampleswere clustered together in same clade ofParagonimus westermani.But the phylogenetic trees could not revealed geographically genetic relationships of D.dendriticum isolates in this province.These findings will provide basic information for further study of molecular epidemiology and control ofD.dendriticum infection in this province as well as in China.
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