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Escherichia coli (E.coli) strains have been assigned membership to various phylogenetic groupings based on multilocus enzyme electrophoresis (MLEE) or ribotyping.Similar phylogenetic groupings can also be generated with a multiplex PCR based on only three gene markers developed by Clermont et al.(2000).None of these approaches specifically address the role virulence genes may play in determining the pathogenic status of E.