Analysis of three compouds in Flos Farfarae by Capillary Electrophoresis with Large-volume sample st

• 作 者: Zeng-YanHAO;Yu-JiaoWU;Wen-YanZHAO;Guo-YanWANG;An-JiaCHEN;
• 机 构: College of Pharmacy,Shanxi Medical University,Taiyuan 030001,China
• 出 处: 第八届全国微全分析系统学术会议、第三届全国微纳尺度生物分离分析学术会议暨第五届国际微化学与微系统学术会议
• 发表日期: 2013年期

　　A technique combining an on-capillary concentration method known as large-volume sample stacking and high-efficiency CE separation has been developed to analyze and detect three compounds in Flos Farfarae,such as rutin,quercetin and chlorogenic acid.The optimized conditions were as follows: the buffer: 40mmol/L NaH2P04 + 40 mmol/L Borax + 30% methanol (pH 8.0); the sample hydrodynamic injection of up to 4 s at 5 p.s.i..Significantly selective and sensitive improvements were observed and about 100-fold enrichment was achieved (based on peak area).This method was successfully applied to determine the three compounds in Flos Farfarae.The curve of peak response versus concentration was linear from 20 to 400μg/mL-16.5~330μg/mL and 25 to 500μg/mL.The regression coefficients were 0.9998,0.9999 and 0.9991,respectively.The detection limit of the current technique for was 0.04 μg/mL based on the signal-to-noise ratio of 3.