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Objective To evaluate the renoprotection effects of non-steroidal anti-inflammatory drugs (NSAIDs) in renal ischemia reperfusion injury (1RI),and the COX1/2 blockade association by Indomethacin (IMT) in mice model.Methods:IMT were administrated after left renal pedicle is clamped at four doses of IMT (1,3,5 and 7 mg/kg) by intraperitoneal injection.Blood and kidney samples were collected 24 hours after isehemia reperfusion injury.The renal functions and the cytokines were assayed creatinine in the serum by using ELISA kits.Kidney samples were analyzed by hematoxylin and eosin (HE) and immunohistochemistry staining.Results The mice administered with IMT (5mg/kg) had a marked reduction in serum creatinined and significantly less tubular damage.The tumor necrosis factor α (TNF-α) activity in rena/homogenates and interleukin 6 (IL-6) activity in serum had a marked reduction at doses of IMT (5,7mg/kg).The administrations of dosages (3,5mg/kg) had a marked reduction in the ratio of Thromboxane B2 to 6-keto Prostaglandin F1 α.COX 1 and 2 staining were weaker in IMT (Tmg/kg) groups than the other groups.Conclusion:There was a dose-response on IMT function of renal ischemia reperfusion injury in mice,and IMT had a protective effect in a certain dose range.The effects of IMT on mice ischemia reperfusion injury was related to COX 1 and 2 blockades.