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Induced multipotent stem (iMS) cells areorigi-natedfromsomaticcellsandbecomemultipotentbygenetic and/or epigenetic modifications.Previous studieshaveshown that the fish oocytes extracts (FOE) can induceskin fibroblastcellsintoiMScells.Inthisstudy, weaimto determine whether FOE can similarly inducemouseperipheral blood mononuclear cells (PBMCs) into theiMSstate and if so, whether they can survive longer whentheyaretransplantedintotheirradiationfemalemice.PBMCsofGFP-transgenic male mice were cultured andtransientlyreprogrammed by FOE.They were deemed reachingthe iMSstateafterdetectionofexpressionofstemcellmarkers.TheiMS-like PBMC sweretransplantedintofemaleC57BL mice by tail vein injection.The spleen wet weights aswell asnumbersofcoloniesoftherecipientrnicewereexamined.The results showed the spleen wet weights and numbersofspleencoloniesofFOE-inducedgroupwereallsignificantly higher than those of the non-induced group and nega-tive control group.On day 90 after transplantation,FISH analysis detected the presence of Y chromosome inthe inducedgroup,butnotoftheothergroups.Thecurrent findings demonstrate that FOE-induced PBMCs are ableto survive longer in irradiated femalemice.