仿生髓核组织工程支架材料对体外培养髓核细胞生长增殖的影响

• 作 者: 李长青;周跃;罗刚;张传志;
• 机 构: 第三军医大学新桥医院骨科,重庆,400037
• 出 处: 第七届全国脊柱脊髓损伤学术会议
• 发表日期: 2007年期

　　Objective:To examine the effect on the growth and proliferation of nucleus pulposus cell cultured in vitro induced by CHCS scaffold for nucleus pulposus tissue engineering.Methods:Primary nucleus pulposus cells cultured in vitro were harvested,and then planted into the culture flasks,in which CHCS scaffold had been put.After the cells were cultured for 10 days,the numbers were counted.The proliferation ratio and the relative proliferation were calculated.The DNA contents of the cells were examined by Hoechst 33258 staining.