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Indigenous oil-degrading bacteria play an important role in efficient remediation of polluted marine environments.In this study,we investigated the diversity and abundance of indigenous oil-degrading bacteria and functional genes in crude oil-contaminated seawater of Dalian coast.Bacteria of Alcanivorax,Marinobacter,Novosphingobium,Rhodococcus and Pseudoalteromonas were found predominant oil-degrading bacteria in the polluted seawater in situ.In addition,bacteria belonging to Algoriphagus,Aestuariibacter,Celeribacter,Fabibacter,Zobellia,Tenacibaculum,Citreicella,Roseivirga,Winogradskyella,Thioclava,Polaribacter and Pelagibaca were confirmed to be the first time as an oil-degrading bacterium.The indigenous functional enzymes,including AlkB or PAH-RHDα coding genes from Gram-positive (GP) and negative bacteria(GN),were revealed and quite diverse.The two-thirds of all the AlkB sequences were closely related to widely distributed Alcanivorax and Marinobacter isolates; Almost all RHDαGN sequences were closely related to an uncultured bacterium; In RHDαGP sequences,the naphthalene dioxygenase sequences from Rhodococcus and Mycobacterium species were most abundant.Results of this report indicate that oil-degrading bacteria were quite diverse bacteria compared to the non-polluted areas,which were stimulated by the occurrence of spilled oil to a high population,and play an important role in intrinsic bioremediation in the coastal area of Yellow sea.