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A total of 276 endophytic bacteria were isolated from the root nodules of soybean(Glycine max L.)grown in 14 sites of Henan province in China.The inhibitory activity of these bacteria against a pathogenic fungus Phytophthora sojae 01 was screened in vitro.Six strains with more than 63%inhibitory activities were further characterized by optical epifluorescence microscopic observation,sequencing,and phylogenetic analysis of 16S rRNA gene,potential plant growth-promoting properties analysis,and plant inoculation assay.