Isolation of a Bluetongue virus group-specific monoclonal antibody and application to a diagnostic c

• 作 者: Q. Y. Xu E. C. Sun Y. F. Feng S. Lv Q. Zhang H. X. Wang D. L. Wu
• 机 构: TheKeyLaboratoryofVeterinaryPublicHealth,MinistryofAgriculture,StateKeyLaboratoryofVeterinaryBiotech
• 出 处: 中国畜牧兽医学会兽医病理学分会第二十一次学术研讨会暨中国病理生理学会动物病理生理学专业委员会第二十次学术研讨会
• 发表日期: 2015年7期

Object:Bluetongue (BT) disease is caused by infection with Bluetongue virus (BTV).The mortality ratecan be as high as 50–70 ％in certain sheep breeds.Here,we describe the BTV VP7 group-specific Mab4H7 and identify the key amino acid residues in the VP7 protein that contribute to a conformational epitope.And we developed a competitive enzyme-linked immunosorbent assay (cELISA) using Mab 4H7 andrecombinant BTV VP7 protein.