【摘 要】
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The hepatitis B virus (HBV) remains to be a global health threat since the chronic HBV infection may lead to liver cirrhosis or cancer.Current antiviral therapies with nucleoside analogues can inhibit
【机 构】
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State Key Laboratory of Virology and Modern Virology Research Center,College of Life Sciences,Wuhan
【出 处】
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第十一届全国病毒学学术研讨会暨第六届武汉现代病毒学国际研讨会
论文部分内容阅读
The hepatitis B virus (HBV) remains to be a global health threat since the chronic HBV infection may lead to liver cirrhosis or cancer.Current antiviral therapies with nucleoside analogues can inhibit the replication of HBV,but do not disrupt the already existing HBV cccDNA.The newly developed clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR-associated 9 (Cas9) system is a powerful tool to target cellular genome DNA for gene editing.In order to investigate the possibility of using CRISPR/Cas9 system to disrupt the HBV DNA templates,we designed eight gRNAs that target the conserved regions of different HBV genotypes,which could significantly inhibit HBV replication both in vitro and in vivo.Moreover,the HBV-specific gRNA/Cas9 could inhibit the replication of HBV of different genotypes in cells and the viral DNA is significantly reduced by single gRNA/Cas9 and cleared by combination of different gRNAs/Cas9.
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