Comparative genomic sequence analysis between a standard challenge strain and a vaccine strain of du

• 作 者: Chenghuai Yang Qihong Li Junping Li Guangchuan Zhang Huijiao Li Yecai Xia Hanchun Yang Kangzhen Yu
• 机 构: Key Laboratory of Animal Epidemiology and Zoonosis of Ministry of Agriculture,College of Veterinary
• 出 处: 第五届中国兽药大会(中国畜牧兽医学会动物药品学分会2014年学术年会、中国畜牧兽医学会生物制药学分会暨中国微生物学会兽医
• 发表日期: 2014年6期
• 关键词: virulent duck enteritis virus genome comparative

　　Here we present the complete genomic sequence of the Chinese standard challenge strain (CSC) of duck enteritis virus (DEV), which was isolated in China in 1962. The DEV CSC genome is 162,131 bp long and contains 78 predicted open reading frames (ORFs). Comparison of the genomic sequences of DEV CSC and DEV live vaccine strain K at passage 63 (DEV K p63) revealed that the DEV CSC genome is 4040 bp longer than the DEV K p63 genome, mainly because of 3513-bp and 528-bp insertions at the 5 and 3 ends of the unique long segment, respectively. At the nucleotide level, 63 0f the 76 0RFs in the DEV CSC genome were 100% identical to the ORFs in the DEV K p63 genome. Two ORFs (UL56 and USlO) had frame-shift mutations in the C-terminal regions, while LORF5 was unique to the DEV K p63 genome. It is difficult to assign attenuated virulence to changes in specific genes. However, the complete DEV CSC genome will further advance our understanding of the genes involved in virulence and evolution. The DEV CSC genome sequence has been deposited in GenBank under accession number JQ673560.