【摘 要】
：
An efficient regeneration and transformation system was established and optimized for adzuki bean (Vigna angularis Willd.Ohwi & Ohashi).6-Benzylaminopurine at 5 mg l-1 was used to increase adventitiou
【机 构】
：
Institute of Vegetable Crops, Jiangsu Aecademy of Agricultural Sciences, Nanjing210014, China
论文部分内容阅读
An efficient regeneration and transformation system was established and optimized for adzuki bean (Vigna angularis Willd.Ohwi & Ohashi).6-Benzylaminopurine at 5 mg l-1 was used to increase adventitious bud induction frequency.The highest frequency of shoot elongation was 92.8% when using a medium composition of MS salts combined with 0.1 mg l-1 IAA, 0.5 mg l-1 GA3, 1.0 mg l-1 zeatin-fiboside, 50 mg l-1 aspartic acid and 50 mg l-1 glutamic acid.In vitro rooting was 100% when shoots were cultured on the solid MS medium supplemented with 1.0 mg l-1 NAA.Reproducible transformation of epicotyl explants was developed using the A.tumefaciens EHA105 strain.Using a concentration of 40 mg l-1 acetosyringone, 20 mM MES and 5 mg l-1 6-benzylarninopurine in co-cultivation medium a transformation efficiency of 12.6% was attained.Using this transformation protocol, we obtained transgenic adzuki bean plants resistant to soybean mosaic virus by introducing the V.angularis VaPR3 gene.
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