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OBJECTIVE To study the effects and mechanisms of 3-(5-hydroxymethyl-2-furyl)-1-benzylindazole(YC-1) on induction of apoptosis in activated hepatic stellate cells(HSCs).METHODS HSC cella were treated with YC-1 for 24 h before analysis.MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl) assay was used to detect the cellular viability.HSC cells were treated with different concentration of YC-1 for 24 h.Apoptosis of HSC was studied in the presence of known apoptotic agents.The PI3K/Akt dependent pathways, NF-κB activation and expression of the antiapoptotic proteins caspase-3 were evaluated.Western blotting analysis were performed.RESULTS Activated HSC are proliferative and fibrogenic, with accumulation of extracellular matrix (ECM), including α-smooth muscle actin (α-SMA) and type Ⅰ collagen.Pro-caspase-3 expression was siguificantly decreased in HSC-T6 cells treated with YC-1.And YC-1 decreased nuclear factor κB (NF-κB) expression in activated HSC cell nuclear.Meanwhile, YC-1 decreased pAkt and piκBα expression in activated HSC cells in a time-dependent manner.Conclusions Our data indicate that YC-1 can efficiently inhibit cell apoptosis in hepatic stellate cells.In summary, YC-1 play an important role in controlling hver fibrosis resolution by regulating the apoptosis of HSC.YC-1 would be a potential therapeutic medicine for patients with hepatic fibrosis.