ICPMS Analysis with New SEC Column for Protein Combined with Metal and Selenoamino Acids Present in

• 作 者: Gaiyun Song Minjie Zeng Kazunori Iwata Yoshimitsu Ogra
• 机 构: ShodexChinaCo.,Ltd.18F,211ShiMenYiRoad,JingAn,Shanghai,ChinaShodexChinaCo.,Ltd.18F,211ShiMenYiRoad,J
• 出 处: 第八届全国微全分析系统学术会议、第三届全国微纳尺度生物分离分析学术会议暨第五届国际微化学与微系统学术会议
• 发表日期: 2013年5期
• 关键词: Micro Column Selenoamino Acids LC-ICP/MS Trace analysis Metalloprotein

　　Metals perform many functions inside organisms to sustain life.These metals are generally present in very small amounts,requiring high sensitivity determinations.To diagnose a congenital metal metabolic disorder,in particular,it is necessary to differentiate between free metals and those combined with proteins.We introduce an LC-ICP/MS method using a new size exclusion chromatography (SEC) mode column to fulfill that specific requirement.We have developed a new SEC micro column (I.D.: 0.3-1 mm) to ensure high-sensitivity determination.Using ICP/MS analysis with this new column,we were able to determine,with high sensitivity,trace amounts of metals in organisms.We explain methods for determining selenoamino acids (derivatives of essential amino acids) and Cu-metallothionein that relates to a congenital metabolic disorder.